Study on the feasibility of a cell-culture inoculation test in the detection and isolation of street rabies virus
10.3760/cma.j.issn.0254-6450.2009.06.018
- VernacularTitle:细胞培养法检测和分离狂犬病街毒的可行性研究
- Author:
Xia WANG
1
;
Ge-Lin XU
;
Jie WU
;
Jing LUO
;
Jia-Hong ZHU
;
Jian-Jun ZHOU
;
Nan-Feng SHI
;
Qing TANG
;
Yong-Zhen ZHANG
;
Jian-Rong TANG
;
Tai-Cai WU
;
He-Tian MING
;
Rui-Hua SHEN
;
Huan-Chun CHEN
Author Information
1. 湖北省武汉市生物制品研究所
- Keywords:
Street rabies virus;
Cell-culture inoculation test;
Detection
- From:Chinese Journal of Epidemiology
2009;30(6):611-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus. Methods Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains. Results 33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%. Conclusion MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus.
