Study on the molecular characteristics of multidrug-resistant Acinetobacter baumannii

VernacularTitle:多重耐药鲍曼不动杆菌的分子流行特征分析
Author: Jing-Rong CAO ¹ ; Xing WEI ; Zhong-Qiang YAN ; Ding-Xia SHEN ; Yan-Ping LUO
Author Information

1. 解放军总医院
Keywords: Acinetobacter baumannii; Multidrug-resistant; Epidemiology,molecular
From: Chinese Journal of Epidemiology 2009;30(8):832-835
CountryChina
Language:Chinese
Abstract: Objecfive To investigate antibiotic resistance,carbapenemase genotype and the molecular epidemiology of multidrug-resistant Acinetobacter baumannii (Aba) collected from 3 military hospitals in China.Methotis The minimum inhibitory concentrations (MIC) were examined by ager dilution method.Genotypes of carbapenemases were amplified by multiplex PCR and its products were sequenced.PCR was used to detect per gene,Homology of the resistant isolates was analyzed by pulse-field gel electrophoresis(PFGE).Results Among the 64 MDRA strains,78.1%(50)strains possessed blaOXA-23 gene,89.1%(57) carried Class 1 integrase gene,39.1% (25) with blaPER-1 gene,and 1 strain with blaOXA-58-like gene.PFGE showed that 13(A,B,C,D,E genotype) different clones were identified in these strains.A,B,and U clones were the predominant clones in three hospitals,respectitively.Conclusion OutbreaEs of muitidrug-rcsistant Aba occurred at 3 military hospitals with the most prevalent carbapenemase as OXA-23 enzyme.OXA-58 type of carbapenemase and per-1 in Aba were also isolated.