Airway inflammation induced by Poly(I:C) stimulation in the late stage of respiratory syncytial virus infection in mice and its mechanism.
- Author:
Ke-Ting ZHAO
1
;
Xiao-Ru LONG
;
Wei LI
;
Jun XIE
;
Luo REN
;
Yu DENG
;
Xiao-Hong XIE
;
Na ZANG
;
Li-Jia WANG
;
En-Mei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Asthma; etiology; Bronchoalveolar Lavage Fluid; chemistry; Female; Lung; pathology; Matrix Metalloproteinase 9; analysis; Mice; Mice, Inbred BALB C; Poly I-C; pharmacology; Respiratory Syncytial Virus Infections; complications; Tissue Inhibitor of Metalloproteinase-1; analysis
- From: Chinese Journal of Contemporary Pediatrics 2016;18(5):455-459
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the pathogenic mechanisms of airway inflammation and recurrent wheezing induced by recurrent respiratory virus infection after respiratory syncytial virus (RSV) infection.
METHODSSixty-four female BALB/c mice (aged 6-8 weeks) were randomly divided into four groups: control, RSV, Poly(I:C), and RSV+Poly(I:C) (n=16 each). The bronchoalveolar lavage fluid (BALF) was collected on the 3rd day after Poly(I:C) administration, and the total cell number and differential counts in BALF were determined. Hematoxylin-eosin staining was used to observe pulmonary pathological changes. The airway responsiveness was detected. ELISA was used to measure the levels of interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-13 (IL-13), matrix metallopeptidase-9 (MMP-9), and tissue inhibitor of metalloproteinase-1 (TIMP-1) in BALF.
RESULTSCompared with the other three groups, the RSV+Poly(I:C) group had significant increases in the total number of inflammatory infiltrating cells in the airway, airway responsiveness, and MMP-9 level in BALF (P<0.05). The RSV+Poly(I:C) group showed more severe pulmonary tissue injuries compared with the control and RSV groups (P<0.01). Compared with the RSV group, the RSV+Poly(I:C) group showed significant reductions in the levels of IL-4 and TIMP-1 in BALF (P<0.01).
CONCLUSIONSViral re-infection in the late stage of RSV infection may cause an imbalance of MMP-9/TIMP-1 expression and thus contribute to aggravated airway inflammation.