Genotypes of adenoviruses in infants and young children with diarrhea.
- Author:
Qiu-Li ZHANG
1
;
Hong-Bo WANG
;
Yan-Ling WANG
;
Jian-Sheng ZHOU
;
Juan CAI
;
Rui-Rong HE
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; classification; genetics; Child, Preschool; Diarrhea; virology; Female; Genotype; Humans; Infant; Infant, Newborn; Male
- From: Chinese Journal of Contemporary Pediatrics 2016;18(8):718-720
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the prevalence of adenoviruses (AdV) and their genotypes in infants and young children with diarrhea.
METHODSA total of 380 children with diarrhea aged less than 3 years were enrolled. The genomic DNA was extracted from stool and PCR was used to detect AdV. Clone sequencing and genotyping were performed for DNA in AdV-positive specimens.
RESULTSAdV was detected in 24 out of 380 specimens, and the detection rate was 6.3% (24/380). A majority of children with positive AdV were aged 2-3 years. The viral sequence analysis of positive specimens showed that the detection rates of enteric AdV41 and non-enteric AdV were 4.2% (16/380) and 2.1% (8/380), respectively, and among the children with non-enteric AdV, there were 2 with AdV1, 2 with AdV2, 1 with AdV7, 2 with AdV12, and 1 with AdV31.
CONCLUSIONSDiarrhea caused by AdV is commonly seen in children aged 2-3 years, and AdV41 is the major predominant strain.
