Effect of Chinese drugs for supplementing qi-blood and nourishing gan-shen on transforming growth factor beta1, and sex hormone levels in the follicle fluid of women during IVF-ET cycle.
- Author:
Ming-xiao GE
1
;
Jin-yu ZHANG
;
Yan-peng ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Drugs, Chinese Herbal; pharmacology; Embryo Transfer; Female; Fertilization in Vitro; Follicular Fluid; metabolism; Humans; Luteinizing Hormone; metabolism; Transforming Growth Factor beta1; metabolism; Young Adult
- From: Chinese Journal of Integrated Traditional and Western Medicine 2011;31(3):327-330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Chinese drugs for supplementing qi-blood and nourishing Gan-Shen (CD) on transforming growth factor beta1 (TGF beta1) and sex hormone levels in the follicle fluid of women during in vitro fertilization-embryo transfer (IVF-ET) cycle.
METHODSEighty-six women undergoing IVF-ET were randomly assigned to two groups, 45 in the CD group and 41 in the control group. All received the standard regimen for promoting ovulation, but to women in the CD group, 1-week treatment of Cuhuangti Granule was administered during the period of gonadotropin releasing hormone agonist (GnRH-a) down-regulation, and Jinghou Zengzhi Granule was given from time of ovulation promoting with Gn to the day of HCG administration. On the day of oocyte retrieval, TGFbeta1 was detected using enzyme linked immunosorbent assay (ELISA), estradiol (E2), progestone (P), and luteinizing hormone (LH) detected by radioimmunoassay.
RESULTSFollicle fluid contents of TGFbeta1 and LH in the CM group (3.25 +/- 1.11 pg/L and 0.89 +/- 0.45 IU/L) were obviously higher than those in the control group (2.21 +/- 1.08 pg/L and 0.57 +/- 0.42 IU/L, both P < 0.05).
CONCLUSIONChinese drugs for supplementing qi-blood and nourishing Gan-Shen could significantly improve TGFbeta1 and LH levels in the follicle fluid of women, thus enhancing the embryo implantation rate.
