The cytoskeleton and immigration of laryngeal squamous carcinoma cell affected by Fascin-1 expression.
- Author:
Jian ZON
1
;
Fei CHEN
;
Jiang WU
;
Wen LI
;
Lin LOU
;
Shixi LIU
;
Hui YANG
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, China. zoujian926@163.com
- Publication Type:Journal Article
- MeSH:
Carcinoma, Squamous Cell;
metabolism;
ultrastructure;
Carrier Proteins;
genetics;
metabolism;
Cell Line, Tumor;
Cell Movement;
Cytoskeleton;
metabolism;
ultrastructure;
Down-Regulation;
Humans;
Laryngeal Neoplasms;
metabolism;
ultrastructure;
Microfilament Proteins;
genetics;
metabolism;
RNA, Small Interfering;
genetics
- From:
Journal of Biomedical Engineering
2010;27(5):1034-1038
- CountryChina
- Language:Chinese
-
Abstract:
This study sought to detect the effect of Fascin-1 expression on the cytoskeleton and immigration of laryngeal squamous carcinoma cell. In the experiment, Fascin-1 expression in Hep-2 cells was inhibited by small interfering RNA. The cytoskeleton of Hep-2 cells was observed with the use of laser scanning confocal fluorescence microscope. Millicell insert was applied to detect the immigration of Hep-2 cells in vitro. The results showed that the integrity of cytoskeleton in Hep-2 cells was broken with the down-regulation of Fascin-1 expression and the immigration ability was decreased significantly (P < 0.05). The inhibiting ratio of cell immigration was 44.6 +/- 6.3%. In conclusion, inhibition of Fascin-1 expression in Hep-2 cells could break the integrity of cytoskeleton and decrease the ability of cellular immigration.
