Construction and expression of repeats of HER-2 mimetic peptide.
- Author:
Lili ZHAO
1
;
Lin MENG
;
Beihai JIANG
Author Information
1. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China.
- Publication Type:Journal Article
- MeSH:
Breast Neoplasms;
metabolism;
Cancer Vaccines;
biosynthesis;
immunology;
Epitopes;
Escherichia coli;
genetics;
metabolism;
Female;
Gene Expression;
Humans;
Peptides;
genetics;
immunology;
metabolism;
Receptor, ErbB-2;
biosynthesis;
chemistry;
genetics;
Recombinant Proteins;
biosynthesis;
genetics;
immunology
- From:
Journal of Biomedical Engineering
2010;27(5):1076-1099
- CountryChina
- Language:Chinese
-
Abstract:
To produce many epitopes of peptide mut, different tandem repeats containing HER-2 mimetic peptide mut were constructed and expressed. The oligonucleotide coding mut sequence was inserted into pET28a(+) to construct the prokaryotic recombinant plasmid pET28a-mutl. The mut coding sequence was repeatedly inserted into the vector in tandem to obtain a series of plasmids pET28a-mut2, pET28a-mut4, pET28a-mut8 and pET28a-mut16. The plasmids were transformed into E. coli. BL21 and induced by IPTG. The recombinant proteins were expressed in E. coli. The binding analysis verified that the interaction between the tandem peptides mut and Herceptin was increased. In conclusion, the tandem peptides consisted of many antigen epitopes, and laid the foundation for the further study of HER-2 peptide vaccine for biotherapy of cancer with HER-2 overexpression.
