The isolation and assessment of Golgi apparatus from gastric cancer cells SGC7901.
- Author:
Tingting HE
1
;
Yongfen YI
;
Yanqing LI
;
Zhong XIAO
Author Information
1. Department of Pathology, Chongqing Medical University, Chongqing 400016, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Golgi Apparatus;
ultrastructure;
Histological Techniques;
Humans;
Stomach Neoplasms;
pathology;
ultrastructure
- From:
Journal of Biomedical Engineering
2010;27(5):1085-1088
- CountryChina
- Language:Chinese
-
Abstract:
The Golgi complex is the central organelle of the secretory pathway and has many complicate functions. The endeavours to isolate and purify the Golgi apparatus from cultured cells will benefit further investigation of Golgi. A large number of gastric cancer cells SGC7901 were cultivated in vitro, then Golgi apparatus were isolated from the cells by differential centrifugation combined with sucrose density gradient ultra-centrifugation. Its purity was characterized biochemically by enzymatic assays, morphologically by electron microscopy (EM) and neutral red supravital staining. Finally the Golgi complex was successfully fractionated from gastric cancer cells SGC7901. The first successful isolation of Golgi apparatus from gastric cancer cells SGC7901 by using ultra-centrifugation will lead to research into the function of Golgi apparatus.
