Association of HLA-A0205 and HLA-A30 with latent autoimmune diabetes in adults.
- Author:
Chunying DENG
1
;
Nanwei TONG
;
Xiujun LI
Author Information
1. Department of Endocrinology, West China Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Age of Onset;
Autoimmune Diseases;
genetics;
Case-Control Studies;
China;
Diabetes Mellitus;
genetics;
Female;
Genotype;
HLA-A Antigens;
genetics;
Humans;
Male;
Polymerase Chain Reaction;
methods;
Polymorphism, Single-Stranded Conformational
- From:
Journal of Biomedical Engineering
2010;27(5):1089-1094
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the association of HLA-A0205 and HLA-A30 with latent autoimmune diabetes mellius in adults (LADA) in Chengdu Hans, 121 subjects (41 cases of LADA, 40 cases of T2DM, and 40 normal controls) were enrolled in the study. The frequencies of HLA-A0205 and HLA-A30 were determined by nested PCR-SSP and direct sequencing, respectively. The allele frequencies of patient groups and of normal controls were compared by chi-square test using SPSS 11.0 (alpha = 0.05). Hardy-Weinberg equilibrium was tested with use of software HWE (alpha = 0.05). Data from the subjects showed: HLA-A0205 was present in 1 patient with LADA and in 1 normal control (2.44% and 2.5%, respectively), HLA-A30 was present in 2 patients with LADA, in 2 patients with T2DM and in 1 normal control (4.87%, 5.0% and 2.5%, respectively). There was no statistically significant difference between the allele frequencies of the three groups. These results suggest that HLA-A0205, HLA-A30 may not be related to LADA in Chengdu Hans. Yet, further studies with larger sample size may be needed to warrant this conclusion.
