Influence of genipin and vitamin E on UCP2 and other correlation factors in non-alcoholic fatty liver disease.
- Author:
Lin LIN
1
;
Xiaoqin GUAN
;
Lijuan WANG
;
Yuanting TANG
Author Information
1. Department of Pathology, Chongqing Medical University, Molecular Medicine and Cancer Research Center, Chongqing 400016, China.
- Publication Type:Journal Article
- MeSH:
Cell Line;
Drug Synergism;
Fatty Liver;
metabolism;
Humans;
Ion Channels;
genetics;
metabolism;
Iridoid Glycosides;
pharmacology;
Iridoids;
Liver;
cytology;
Membrane Potential, Mitochondrial;
Mitochondrial Proteins;
genetics;
metabolism;
NF-kappa B;
genetics;
metabolism;
Non-alcoholic Fatty Liver Disease;
Protective Agents;
pharmacology;
RNA, Messenger;
genetics;
metabolism;
Tumor Necrosis Factor-alpha;
genetics;
metabolism;
Uncoupling Protein 2;
Vitamin E;
pharmacology
- From:
Journal of Biomedical Engineering
2010;27(6):1327-1331
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to detect the effect of genipin and Vitamin E (VitE) on non-alcoholic fatty liver disease. L02 cells were divided into five groups:control group, palmic acid treated group, VitE treated group, genipin treated group, and a combination group. All treatments were terminated at the end of 72 hours. Pathological changes of L02 cells were observed. Mitochondrial membrane potential changes were detected by flow cytometry. MDA, SOD, ALT, AST, GGT, TG in culture medium and expression of UCP2 mRNA and protein in L02 cells were detected. We also studied the effects of genipin and VitE on UCP2 and other related factors such as NF-kappaB and TNF-alpha on the L02 cell model of non-alcoholic fatty liver disease. In combination group, the degree of adipose degeneration of L02 cells mitigated significantly; mitochondrial membrane potential and the level of SOD activity increased; the level of MDA, ALT, AST, GGT, TG and the expression of UCP2, NF-kappaB,TNF-alpha in L02 cells decreased. The use of genipin in combination with VitE can increase mitochondrial membrane potential and markedly relieve the adipose degeneration of liver cells.
