Effects of M-CSF concentration, RANKL concentration and M-CSF preinduction on osteoclastogenesis.
- Author:
Wei DONG
1
;
Jing YU
;
Mengchun QI
;
Yuhong BAI
;
Ruiying LIANG
;
Huaiqing CHEN
Author Information
1. Department of Stomatology, North China Coal Medical University, Tangshan 063000, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cell Culture Techniques;
methods;
Cell Differentiation;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Culture Media;
pharmacology;
Leukocytes, Mononuclear;
cytology;
Macrophage Colony-Stimulating Factor;
pharmacology;
Mice;
Osteoclasts;
cytology;
RANK Ligand;
pharmacology
- From:
Journal of Biomedical Engineering
2010;27(6):1336-1340
- CountryChina
- Language:Chinese
-
Abstract:
This study was directed to the effects of macrophage-colony stimulating factors (M-CSF) concentration, recerptor activator of nuclear factor kappaB ligand (RANKL) concentration and M-CSF preinduction on osteoclastogenesis and the related resorption function. Bone marrow mononuclear cells were isolated and were divided into 4 groups. Group A underwent osteoclastogenic induction with the use of 30 ng/ml M-CSF and 50 ng/ml RANKL, while Group B received 50 ng/ml M-CSF and 100 ng/ml RANKL treatment. Both C and D Group underwent preinduction with the use of 30 ng/ml M-CSF for 3days, and then they were treated with 30 ng/ml M-CSF and 50 ng/ml RANKL, 50 ng/ml M-CSF and 100 ng/ml RANKL, respectively. Osteoclastogenesis was examined by TRAP staining 6 days after induction, and dentin resorption lacunae were detected by Scanning Electron Microscope 9 days after induction. TRAP positive multinuclear cells were observed in all groups of cells, and resorption lacunae were formed in all of them. However, more TRAP positive multinuclear cells were observed and more large resorption lacunae were detected in groups B and D than in groups A and C, respectively. The number of TRAP positive cells, number of resorption lacunae and lacuna areas in groups C and D were also greater than those in groups A and B, respectively. Higher concentration of M-CSF and RANKL and preinduction with M-CSF may benefit osteoclastogenesis and increase resorption function of osteoclast.
