Hopping probe scanning ion conductance microscopy and its applications to topological imaging of live neuronal cells.
- Author:
Xiao LIU
1
;
Xi YANG
;
Xiaofan ZHANG
;
Hujie LU
;
Liping LIU
;
Yanjun ZHANG
Author Information
1. China National Academy of Nanotechnology & Engineering, Tianjin 300457, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
ultrastructure;
Humans;
Ion Channels;
metabolism;
ultrastructure;
Microscopy, Scanning Probe;
instrumentation;
methods;
Molecular Imaging;
methods;
Neurons;
ultrastructure;
PC12 Cells;
Rats
- From:
Journal of Biomedical Engineering
2010;27(6):1365-1378
- CountryChina
- Language:Chinese
-
Abstract:
As a new kind of non-contacting high-resolution scanning probe microscopy, hopping probe scanning ion conductance microscopy (HPSICM) overcomes the disadvantages of the continuous feedback-control scanning mode of the conventional scanning ion conductance microscopy (SICM), which has been restricted to imaging relatively flat biological samples. The basic operation principles of HPSICM were briefly introduced in this paper. Then the high-resolution 3D morphological images of the two live neuronal cell lines, SK-N-SH cells and NGF-differentiated neurite PC12 cells, were respectively acquired in their physiological culture environment using HPSICM. It is demonstrated that HPSICM provides a powerful microscopy for in-depth studying the microstructures and their dynamic changes of live neuronal cells in real time.
