Quantitative detection of matrix metalloproteinase-2 in normal coronal dentine of young people
10.3760/cma.j.issn.1002-0098.2014.04.011
- VernacularTitle:健康青年正常牙本质中基质金属蛋白酶2的定量检测初探
- Author:
Danyang WANG
1
;
Ling ZHANG
;
Fang LI
;
Yujiang CHEN
;
Jihua CHEN
Author Information
1. 西安医学院口腔医学系口腔修复学教研室
- Keywords:
Dentin;
Matrix metalloproteinase 2;
Fluorescent microsphere immunoassay
- From:
Chinese Journal of Stomatology
2014;49(4):244-246
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the distribution and concentration of matrix metalloproteinases-2 (MMP-2) in different dentin depth of premolar and molar of young people.Methods Freshly extracted human premolars and molars (aged between 20-30) were sectioned to 1.5 mm thick slices along the longitudinal axis of the tooth separately.Enamel and pulp of each slice was removed,and then the premolar and molar slices were respectively divided into two subgroups according to superficial or deep dentin and pulverized to fine powder.After dentin protein was extracted,the concentrations of MMP-2 in different tooth were detected using fluorescent microsphere immunoassay.Results The content of MMP-2 in superficial layer dentin of premolar was (0.022 ± 0.006) ng/mg.The content of MMP-2 in deep layer dentin of premolar was (2.087 ± 0.090) ng/mg.The content of MMP-2 in superficial layer dentin of molar was (0.336 ± 0.037) ng/mg.The content of MMP-2 in deep layer dentin of molar was (3.312 ± 0.308) ng/mg.Conclusions MMP-2 exists in human coronal dentin.In the same type of teeth of young people,the concentration of MMP-2 in deep dentin was significant higher than those in superficial dentin.In the same dentin depth,the concentration of MMP-2 in molar was significant higher than those in premolar.
