Study of peroxisome proliferator-activated receptor-γcoactivator-1α expression and cytoapoptosis in masseter muscles of unilateral chewing rat
10.3760/cma.j.issn.1002-0098.2014.07.006
- VernacularTitle:偏侧咀嚼大鼠咬肌中过氧化物酶体增殖物受体γ共激活因子1α及细胞凋亡的变化
- Author:
Yingying YANG
1
;
Tingting DING
;
Qingting WU
;
Jingjing KONG
;
Dong QI
;
Ping JI
Author Information
1. 山东大学口腔医学院修复科·山东省口腔生物医学重点实验室
- Keywords:
Masseter muscle;
Peroxisome proliferator-activated receptors;
Apoptosis
- From:
Chinese Journal of Stomatology
2014;49(7):408-411
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of peroxisome proliferator-activated receptor-γ coactivator-1 α (PGC-1 α) mRNA and cytoapoptosis in the rats' masseter muscle which had been influenced by unilateral chewing,and to explore the theoretical foundation of changes in masticatory muscles induced by unilateral chewing.Methods The animal models were established by extracting the Wistar rats' left maxillary molars.Thirty-six female Wistar rats were randomly divided into four groups of 2,4,6 and 8 weeks,nine each.In each group there were six rats with molar extracted and three as control.The Ca2+ level was detected by atomic spectrophotometric method.The relative expression of PGC-1α mRNA was detected by real-time fluorescent quantitative PCR.The apoptosis index was detected by Hoechst staining.Results The Ca2+ level in the muscle on the extraction side were significantly higher than that in the controls in the beginning stage of unilateral chewing,and reached the peak at the 4th week [(43.62 ± 2.36) μg/g].The relative expressions of PGC-1α increased from the beginning and reached the maximum level at the 4th week [extraction side:(1.57±0.10); non-extraction side:(1.92±0.06)],while the relative expressions of PGC-1α in 6 and 8 weeks decreased gradually [extraction side:(1.06 ±0.08),(1.08 ±0.07); non-extraction side:(1.09±0.10),(1.11 ± 0.08)].The changes of apoptosis index on non-extraction side increased continually and peaked at the 6th week [(38.56± 1.64)％].Conclusions PGC-1α and cytoapoptosis played important roles in different stages of tissue remodeling induced by unilateral chewing.
