Expression of nucleotide-binding oligomerization domain1, nuclear factor-kappa B and human beta-defensins in candidal albicans leukoplakia
10.3760/cma.j.issn.1002-0098.2014.08.004
- VernacularTitle:白色念珠菌白斑中核苷酸结合寡聚化结构域1、核因子κB及人β-防御素的表达
- Author:
Yajie QIAN
1
;
Wenhui JIANG
;
Xiang WANG
;
Ning DUAN
;
Xiaofeng HUANG
;
Wenmei WANG
Author Information
1. 210008,南京大学医学院附属口腔医院·南京大学口腔医学院口腔黏膜病科
- Keywords:
Candida albicans;
NF-kappa B;
Beta-defensins;
Leukoplakia,oral;
Nucleotide-binding oligomerization domain 1
- From:
Chinese Journal of Stomatology
2014;49(8):464-469
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of nucleotide-binding oligomerization domain 1 (NOD1),nuclear factor-kappa B(NF-κB) and human beta-defensins in candidal albicans leukoplakia and to investigate the effect of candida albicans infection on key proteins in NOD 1 signaling pathway and the expression of human beta-defensin.Methods Forty cases of oral leukoplakia samples were collected and stained by hematoxylin-eosin staining,periodic acid-Schiff staining,silver staining and immunohistochemical methods.Nineteen samples were positive with these four methods and judged as candidal albicans leukoplakia,and the other twenty-one samples judged as leukoplakia without candidal albicans infection.Western blotting was used to detect the expressions of NOD1 and NF-κB in these forty samples.In addition,the immunohistochemical method was adopted to investigate the relationship between NOD 1,NF-κB,human beta-defensin 1,2,3 expressions and candida albicans.Results The positive rate of candida albicans in oral leukoplakia was 48％(19/40).The expressions of NOD1 and NF-κB in the candida albicans leukoplakia were lower than that in leukoplakia without candida albicans infection.The mean optical density value of NOD 1,NF-κB,human beta-defensin 1,2,3 in candidal albicans leukoplakia were 0.25±0.01,0.30±0.02,0.35±0.02,0.42±0.03,0.36±0.02 respectively,which were significantly lower than that in leukoplakia without candida albicans infection (0.31±0.02,0.47±0.03,0.42±0.02,0.53±0.04,0.47±0.03) (P＜0.05).Conclusions By inhibiting the NOD1 signaling pathway,candida albicans infection may reduce the expression level of human beta-defensin 1,2,3 in oral leukoplakia.
