Study on the in vitro release behavior of bovine serum albumin from calcium phosphate coating on pure titanium surface
10.3760/cma.j.issn.1002-0098.2014.09.007
- VernacularTitle:纯钛表面仿生钙磷涂层中牛血清白蛋白的释放行为研究
- Author:
Xiaojing ZHU
1
;
Yan WANG
;
Hui ZHANG
;
Wei TENG
;
Chengyun NING
;
Huade ZHENG
Author Information
1. 中山大学光华口腔医学院·附属口腔医院口腔修复科·广东省口腔医学重点实验室
- Keywords:
Titanium;
Biomimetic Materials;
Membranes,artificial;
Serum albumin,bovine
- From:
Chinese Journal of Stomatology
2014;49(9):540-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the incorporation rate and release behavior of bovine serum albumin(BSA) incorporated into the calcium phosphate coating by biomimetic deposition,as well as the physical and chemical properties of the hybrid coating,and to provide experimental basis for the fabrication of growth factor/biomimetic calcium phosphate coating and exploration for the loading/release behavior of growth factors.Methods Pure titanium specimens were immersed into saturated calcium phosphate solutions(SCP) containing no BSA(controlled group) and 3 different concentrations of BSA(experimental groups):1,10 and 100 mg/L.Biomimetic calcium phosphate coating was formed on titanium surface and BSA was incorporated into the coating through co-deposition.The topography of the specimen was observed using scanning electron microscopy(SEM).Chemical structure and phase composition of coatings were detected by Fourier infrared spectroscopy(FTIR) analysis and X-ray diffraction(XRD) respectively.BSA incorporation rate and release profile were determined by bicinchoninic acid protein assay kit.Results The biomimetic calcium phosphate coating was mainly composed of hydroxyapatite and octacalcium phosphate.BSA was successfully incorporated into the calcium phosphate coatings in all the 3 experimental groups.With the increase of BSA concentration,plate-like units of the coatings were turned into small grid structure.BSA incorporation rates of the three experimental groups were(72.4 ± 2.4)％,(62.3 ± 0.9)％ and (42.2 ± 1.7)％ respectively.The in vitro release test showed that all three BSA release profiles could be divided into two significant different stages:early burst release stage and later sustained release stage.The amount of BSA release of the 3 experimental groups in 24 h and 30 d were (1.57±0.09),(8.82±0.93),(140.24±3.12) μg,and (2.39±0.29),(14.39±0.70),(151.06±2.00) μg respectively.Conclusions Biomimetic calcium phosphate coating can be used as an effective carrier for protein.BSA concentration has an impact on the incorporation rate and release speed of BSA from the calcium phosphate coating.Favorable BSA incorporation rate and release behavior can be obtained at BSA concentration of 10 mg/L.
