Quantitative determination of six coumarins in Angelica dahurica var. formosana by RRLC-UV.
- Author:
Rui DENG
1
;
Jing ZHANG
;
Weizao LUO
;
Lin ZHOU
;
Haijie ZHENG
;
Yi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Angelica; chemistry; Chromatography, High Pressure Liquid; instrumentation; methods; Chromatography, Reverse-Phase; methods; Coumarins; analysis; Plant Extracts; analysis; isolation & purification
- From: China Journal of Chinese Materia Medica 2010;35(23):3184-3187
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RRLC-UV method for simultaneous determination of oxypeucedanin hydrate, bergapten, oxypeucedanin, imperatorin, cnidilin and oxypeucedanin in Angelica dahurica var. formosana.
METHODThe analysis was carried out on a Agilent Zorbax SB-C, (4.6 mm x 100 mm, 1.8 microm) column eluted with the mobile phases of methanol (A)-water (B) in gradient elution. The flow rate was 1.0 mL x min(-1), and the UV detection wavelength was set at 310 nm.
RESULTAll calibration curves of six coumarins showed good linearity regression within test ranges (r > 0.9998), and the overrall recoveries were in the range of 99.42%-101.4%, with RSD less than 2.4%.
CONCLUSIONThe method is simple, sensitive, reliable and reproducible which can be used for the quality study of Angelica dahurica var. formnosana.
