Determination of 5-hydroxy-7-methoxyflavan in Daemonorops draco by HPLC.
- Author:
Meihong FU
1
;
Jing FANG
;
Hongjun YANG
;
Zhuju WANG
;
Liying TANG
;
Luqi HUANG
;
Lan YANG
;
Dong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Arecaceae; chemistry; Chromatography, High Pressure Liquid; methods; Flavanones; analysis; Plant Extracts; analysis
- From: China Journal of Chinese Materia Medica 2010;35(23):3192-3193
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for the determination of 5-hydroxy-7-methoxyflavan in Daemrnonorops draco by HPLC.
METHODDikma Diamonsil C15 column(4. 6 mm x 150 mm, 5 microm) was used. The mobile phase consisted of (60:40). The temperature of column was 30 degrees C. The flow rate was 1:0 mL x min(-1) and the detection wavelength was at 210 nm.
RESULTThe calibration curve was in good linearity with in the range from 0.01 to 0.10 microg (r = 0.9999). The average recovery was 100.1% (n=6).
CONCLUSIONThe method is simple, rapid, as well as precise and reliable, and can be used for the determination of 5-hydroxy-7-methoxyflavan in D. draco.
