Icariin promotes osteogenic differentiation of rat bone marrow stromal cells in vitro.
- Author:
Yuankun ZHAI
1
;
Baofeng GE
;
Huiping MA
;
Leiguo MING
;
Zhifeng LI
;
Guozheng CHENG
;
Jian ZHOU
;
Keming CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; genetics; metabolism; Animals; Bone Marrow Cells; cytology; drug effects; metabolism; Calcification, Physiologic; drug effects; Cell Differentiation; drug effects; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Flavonoids; pharmacology; Insulin-Like Growth Factor I; genetics; metabolism; Male; Osteogenesis; drug effects; Rats; Rats, Wistar; Stromal Cells; cytology; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(23):3219-3222
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of icariin on the osteogenic differentiation of rat bone marrow stromal cells (rBMSCs).
METHODrBMSCs were cultured by adherence screening method. Icariin was supplemented into the culture at 1 x 10(-5) mol x L(-1). The osteogenic differentiation markers including alkaline phosphatase (ALP) activity, CFU-F(ALP) and mineralized bone modulus were compared between the icariin-supplemented group and the control group. Total RNA was isolated and the gene expression of bFGF, IGF-1, Osterix(OSX) and Runx-2 was investigated by RT Real-time PCR.
RESULTIcariin significantly improved ALP activity, CFU-F(ALP) amounts and mineralized modulus. It also can enhance the mRNA level of bFGF, IGF-1, Osterix and Runx-2.
CONCLUSIONIcariin enhances the osteogenic differentiation of rBMSCs significantly, which suggested that icariin has the potentiality to be a new drug of anti-osteoporosis or fracture healing.
