OBJECTIVETo optimize the manufacturing process of the total flavonoid from Chrysanthemum morifolium and Sophora japonica, and establish the quality control methods for total flavonoid and its indicative constituents.

METHODThe solvent for extraction was investigated and L9 (3(4)) orthogonal experiment was used to optimize the extraction process of the total flavonoid. Colorimetric method was used to determine the content of total flavonoid, and HPLC was used to determine the content of the indicative constituents.

RESULTThe optimized manufacturing process of the total flavonoid was that the prescribed crude drugs were extracted by using 70% ethanol for 3 times, 1 h each time. The extract was concentrated to the final concentration of 0.5 g x mL(-1), and was subjected to HPD-600 macroporous resin column chromatography at the weight ratio 1 : 0. 5 of crude drugs to macroporous resin. The sample was eluted with water in the volume of 10 times of the resin's, and then was desorpted with 85% ethanol in the volume of 5 times of the resin's. The content of total flavonoid was determined as 64.98%-66.79%, and the content of indicative constituents was determined as 5.87% - 6.93% for luteolin-7-O-glucoside and 14.09%-16.62% for rutin.

CONCLUSIONExtracting with aqueous alcohol in combination with purifying with macroporous resin column chromatography is an efficient way to prepare the total flavonoid from Ch. morifolium and S. japonica, and the colorimetric and the HPLC methods are useful for its quality control.