Influence of betulinic acid on proliferation, migration, cell cycle and apoptosis of pancreatic cancer cells.
- Author:
Mengjun JIANG
1
;
Yaoyuan ZHOU
;
Min YANG
;
Rongjun ZHANG
;
Meifen ZOU
;
Gangming CAI
;
Donghui PAN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Cycle; drug effects; Cell Line, Tumor; Cell Movement; drug effects; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation, Neoplastic; drug effects; Humans; Pancreatic Neoplasms; drug therapy; genetics; metabolism; physiopathology; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Triterpenes; pharmacology; bcl-2-Associated X Protein; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(22):3056-3059
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of betulinic acid (BA) on the proliferation, migration, apoptosis and cell cycle of pancreatic cancer cells (BxPC-3) in vitro and elucidate the underlying.
METHODThe effect of BA on the proliferation of BxPC-3 was measured by using sulforhodamine B (SRB) assay. Migratory ability of BxPC3 cells were detected by wound healing assay, and the morphological change was observed with light microscope. The influence of BA on cell cycle of BxPC-3 cells was tested by flow cytometry (FCM). Apoptosis was analyzed by using Hochest33342-PI double staining. Western blot technologies were applied to detect the expression of Bcl-2 and Bax.
RESULTBA exhibited significant cell proliferation and migration inhibition, as well as its potency of inducing apoptosis in BxPC-3 cells in vitro in a dose-dependent manner. The IC50 value for 72 h was 16.54 mg x L(-1). Cell migration was significantly inhibited at 5 mg x L(-1) of BA. Cells treated with BA showed increased cell population in G0 phase, with decreased G2/M phase population. The expression of Bax and Bcl-2 was up and down-regulated respectively in BA-treated BxPC-3 cells in a dose-dependent manner.
CONCLUSIONBA exerted potent effect on growth inhibition, G0 cell cycle arrest and induction of apoptosis in BxPC-3 cells in vitro, possibly associated with the down-regulation of Bcl-2 and up-regulation of Bax expression. The potent antitumor capacity of BA suggested that it could be a promising new anticancer agent in human pancreatic cancer treatment.
