Determination of magnoflorine in Coptidis Rhizoma and Phellodendri Chinensis Cortex by LC-MS.
- Author:
Shengjun FAN
1
;
Dahai YU
;
Yuanqinl GU
;
Mingyu ZHANG
;
Lin ZHANG
;
Guoyu LI
Author Information
1. Department of Pharmacology, Harbin Medical University, Harbin 150086, China.
- Publication Type:Journal Article
- MeSH:
Aporphines;
analysis;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Linear Models;
Mass Spectrometry;
methods;
Time Factors
- From:
China Journal of Chinese Materia Medica
2010;35(24):3322-3324
- CountryChina
- Language:Chinese
-
Abstract:
A rapid and specific high performance liquid chromatography-mass spectrometric method was developed for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis. Samples were extracted by methanol. Agilent Eclipse XDB-C18 ODS column (4.6 mm x 150 mm, 5 microm) was used, and mobile phase was methanol-water (60:40) at a flow rate of 0.8 mL x min(-1). Electrospray ionization model (ESI), and MRM model were used for quantification. The linear range of magnoflorine was 4.352-2720 microg x L(-1). The average recovery was above 98%. The method is simple, sensitive and accurate, it can be used for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis.
