Inhibition of HBV replication and antigen expression by RNA interference against different targets.
- Author:
Bing-qiang ZHANG
1
;
Ying HUANG
;
Ni TANG
;
Ying WU
;
Jun ZHANG
;
Wei-xian CHEN
;
Tong-chuan HE
;
Ai-long HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Gene Expression; Hep G2 Cells; Hepatitis B virus; genetics; metabolism; physiology; Humans; RNA Interference; RNA, Small Interfering; genetics; Transfection; Virus Replication
- From: Chinese Journal of Hepatology 2006;14(9):662-665
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo observe the inhibition of HBV replication and antigen expression by RNA interference aimed at different parts of the HBV genome.
METHODSFollowing the rules of shRNA expression vector design and construction, we constructed seven kinds of sequence specific vectors and two kinds of mutant shRNA expression ones. We then cotransfected those shRNA and HBV expression vectors into HepG2 cells using lipofectamine2000. The level of HBV replication was investigated using Southern blot and the antigen expression using ELISA.
RESULTSThe replication of HBV DNA was inhibited by many shRNAs, especially the ones against P1, S2, C2, S1 and X. The inhibition rate against P1 was as high as 95%. Results obtained with ELISA showed that the shRNAs targeting C2, C1 and S2 had high rates of inhibition to HBsAg.
CONCLUSIONThe replication and antigen expression of HBV could be inhibited by shRNAs aimed at four different open read frames, and higher inhibition rates of HBV replication and surface antigen expression could be obtained by P1 and C2, respectively.
