A study on the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.
- Author:
Hui ZHANG
1
;
Jin-Hui SUN
;
Hong-Lian GENG
;
Lie-Ying FAN
;
Gao-Lin LIU
;
Long-Yi TAN
Author Information
- Publication Type:Journal Article
- MeSH: Autoantigens; metabolism; Cell Line, Tumor; DNA, Viral; Hepatitis B Surface Antigens; Hepatitis B virus; genetics; metabolism; Humans; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; RNA, Viral; Ribonucleoproteins; metabolism
- From: Chinese Journal of Hepatology 2006;14(10):735-737
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.
METHODSFour specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.
RESULTSIn comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.
CONCLUSIONThere is a correlation between La protein and HBV mRNA and the expression of HBV protein.
