Reduced expression of miR-218 and its significance in gastric cancer.
- Author:
Cai-ping GAO
1
;
Zhi-yu ZHANG
;
Gan-hui CAI
;
Wen-zhong LIU
;
Shu-dong XIAO
;
Hong LU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Down-Regulation; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; genetics; metabolism; physiology; Neoplasm Invasiveness; Stomach Neoplasms; genetics; metabolism; pathology; Transfection
- From: Chinese Journal of Oncology 2010;32(4):249-252
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and function of miR-218 in gastric cancer.
METHODSmiR-218 levels were evaluated in 20 non-cardia gastric cancer tissues using TaqMan stem-loop real-time PCR analysis. Pre-miR-218 and anti-miR-218 inhibitor were used to change the miR-218 expression level and examine its effects on cell proliferation, apoptosis, cell cycle and cell invasion.
RESULTSComparing with the corresponding normal tissues, miR-218 expression was significantly reduced in the gastric cancer tissue (P < 0.01). Forced expression of miR-218 increased apoptosis in AGS cells. The proportion of apoptosis cells induced by transfection of pre-miR-218 was greater than that induced by control (21.6% vs. 10.4%, P = 0.032). Pre-miR-218 resulted in a significantly decreased cell growth activity (P < 0.01) and cell invasion (P < 0.05) of AGS cells compared with that of the control.
CONCLUSIONmiR-218 expression is reduced in gastric cancer. miR-218 may function as a tumor suppressor in gastric carcinoma.
