- Author:
Hong XIN
1
;
Yan-hua WANG
;
Hao LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Cells, Cultured; Child; Dose-Response Relationship, Drug; Escherichia coli; chemistry; Fibroblasts; cytology; metabolism; Gingiva; cytology; Humans; Interleukin-11; genetics; metabolism; Interleukin-6; genetics; metabolism; Leukemia Inhibitory Factor; genetics; metabolism; Lipopeptides; pharmacology; Lipopolysaccharides; administration & dosage; isolation & purification; pharmacology; Oncostatin M; genetics; metabolism; Porphyromonas gingivalis; chemistry; RNA, Messenger; metabolism; Signal Transduction; Toll-Like Receptor 2; agonists; Young Adult
- From: Chinese Journal of Stomatology 2012;47(9):523-527
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether signaling through Toll-like receptor-2 (TLR-2) can affect the expression of some cytokines in human gingival fibroblasts.
METHODSThe gingival fibroblasts were isolated and cultured in vivo, divided into blank control group, lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg) group and Escherichia coli (Ec) group. mRNA expression levels were measured by real-time polymerase chain reaction (PCR). The protein expression levels were detected by the enzyme linked immunosorbent assay (ELISA). The data was statistically analyzed by SPSS16.0 software package.
RESULTSLPS from Pg could stimulate the expression of interleukin (IL)-6 and leukemia inhibitory factor (LIF) mRNA and protein, which reached the peak (5.87 ± 0.83) at 10 h, and the expression level increased with the increase of the Pg concentration. IL-11 or oncostatin-M (OSM) mRNA expression was not affected by LPS. After treated with Pg for 48 h, the protein expression of IL-6 and LIF was up-regulated, (962 ± 57) ng/L and (47 ± 18) ng/L respectively.
CONCLUSIONSSignaling through TLR-2 controls the expression of cytokines of IL-6 family in human gingival fibroblasts.