Distribution of anaerobes in periodontal abscess and its resistance to antibiotics.
- VernacularTitle:牙周脓肿专性厌氧菌的分离及其对抗生素耐药性的研究
- Author:
Jun-lin HE
1
;
Li-ying YU
;
Jia-zhen CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Anti-Bacterial Agents; pharmacology; Bacteria, Anaerobic; isolation & purification; Cefmetazole; pharmacology; Clindamycin; pharmacology; Drug Resistance, Bacterial; genetics; Erythromycin; pharmacology; Female; Genes, Bacterial; Humans; Male; Metronidazole; pharmacology; Microbial Sensitivity Tests; Middle Aged; Periodontal Abscess; microbiology; Prevotella; isolation & purification
- From: Chinese Journal of Stomatology 2012;47(12):719-724
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate and culture the predominant anaerobes from the periodontal abscesses, and to test the antibiotic susceptibility and drug resistant genes of the strains.
METHODSThe isolated strains were identified by both API20A biochemical method and polymerase chain reaction (PCR) method. The antibiotic susceptibility test was performed by agar dilution method. The resistant genes of the drug-resistant strains obtained were screened by PCR.
RESULTSThe anaerobes were detected in 48% (28/58) of the samples and Prevotella melaninogenica (Pm) was mostly identified in 43% (12/28). API20A biochemical method had 82% (23/28) agreement with the 16SrRNA method in identification rate. Anaerobes were resistant to metronidazole, clindamycin and cefmetazole. The erythromycin-resistant methylase genes F (ermF) gene was detected in three of eight clindamycin resistant strains. None of them was found coded on bacterial plasmids. However, no metronidazole resistant gene was detected on drug resistant strains.
CONCLUSIONSPm was the predominant species dectected in the periodontal abscess of the patients. The antibiotic agents should be used based on the genotypes and general condition of the patients.
