Therapeutic mechanism of bleomycin A5 on infancy hemangioma: an experimental study
10.3760/cma.j.issn.1002-0098.2013.01.006
- VernacularTitle:平阳霉素治疗婴幼儿血管瘤作用机制的实验研究
- Author:
Peng LI
1
;
Dong-Fan LI
;
Zheng-Tuan GUO
;
Xiao-E XIAO
Author Information
1. 710004,西安交通大学医学院第二附属医院小儿外科
- Keywords:
Hemangioma;
Vascular Malformations;
Bleomycin A5
- From:
Chinese Journal of Stomatology
2013;48(1):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic mechanism of Bleomycin A 5 on infancy hemangioma-Methods After intralesional injection of Bleomycin A5 into the tumor of animal model of infancy hemangioma,the variation of tumor form was and the variation of tumor structure were observed using light microscope and electron microscope,the variation of tumor gene expression spectra was also tested by DNA microarray technique.Results After treatment,the tumor gradually shrunk,hardened,disappeared one month later.The tumor lost appearance of infancy hemangioma and replaced by lamellar collagen fibers and cellular nucleus scattered in the fibers,and almost all cells were necrotic and dissolved.Under electron microscope,only large stretches of dissolved cell could be seen without intact cells and blood vessels,but apoptotic cells and bodies could also be found.The results of DNA microarray analysis showed that 9 genes associated with apoptosis (murine double minute 2,heat-labile enterotoxin B subunit,lymphotoxin B receptor,tumor necrosis factor ligand superfamily 7,tumor necrosis factor receptor superfamily 21,tumor necrosis factor receptor superfamily 1 A,myeloid cell leukemia-1,caspase3),13 genes associated with cell proliferation and cell cycle(cell division cycle27,cell division cycle37,CDC28 protein kinase 1B,cycling B1,cullin 2,cullin 3,cullin 4A,growth arrest and DNA damage-inducible 45A,meiotic recombination 11homolog B,forkhead box Ml,minichromosome maintenance7,antigen identified by monoclonal antibody ki 67,proliferating cell nuclear antigen),and 11 genes associated with cellular stress and toxic reaction (glutathione peroxidase 1,metallothioneins,uperoxide dismutase-1,heat shock protein A1 A,heat shock protein A2,heat shock protein A4,heat shock protein A5,heat shock protein 9B,heat shock protein CA,macrophage migration inhibitory factor,plasminogen activator inhibitor)were up or down regulated more than 2 folds in tumors treated with Bleomcyin A5 compared with controls.Conclusions The therapeutic effect of Bleomycin A5 on infancy hemangioma is the synthetic results of multiple factors.Bleomycin A5 could not only induce apoptosis and inhibit cell proliferation,but also depressed the ability of cell stress and toxic reaction.
