Effect of KH2PO4 on the odonto- and osteogenic differentiation potential of human stem cells from apical papillae.
- Author:
Yan-ping WANG
1
;
Jin-tao WU
;
Zi-lu WANG
;
Yang-yu ZHENG
;
Guang-dong ZHANG
;
Jin-hua YU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; drug effects; Cells, Cultured; Dental Pulp; cytology; Extracellular Matrix Proteins; metabolism; Humans; Osteoblasts; cytology; Osteocalcin; metabolism; Phosphates; pharmacology; Phosphoproteins; metabolism; Potassium Compounds; pharmacology; Sialoglycoproteins; metabolism; Stem Cells; cytology; drug effects; metabolism
- From: Chinese Journal of Stomatology 2013;48(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the effects of KH2PO4 on the odonto- and osteogenic differentiation potential of human stem cells from apical papillae (SCAP) in vitro.
METHODSSCAP were isolated and cultured respectively in alpha minimum essential medium (α-MEM) or α-MEM containing 1.8 mmol/L KH2PO4. Alkaline phosphatase (ALP) activity, alizarin red staining, real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine the odonto and osteogenic potential of SCAP in the two media.
RESULTSSCAP cultured in α-MEM containing 1.8 mmol/L KH2PO4 exhibited a higher ALP activity [(0.370 ± 0.013) Sigma unit×min(-1)×mg(-1)] at day 3 than control group [(0.285 ± 0.008) Sigma unit×min(-1)×mg(-1)] and KH2PO4-treated SCAP formed more calcified nodules at day 5 [(0.539 ± 0.007) µg/g] and day 7 [(1.617 ± 0.042) µg/g] than those in normal medium [(0.138 ± 0.037) µg/g, P < 0.01]. The expression of odonto- and osteogenic markers were significantly up-regulated after the stimulation of KH2PO4 at day 3 and 7 respectively, as compared with control group.
CONCLUSIONS1.8 mmol/L KH2PO4 can promote the odonto and osteogenic differentiation potential of human SCAP.
