Sonic hedgehog signaling enhanced the expression of histone demethylase, lysine-specific demethylase 8 in the head and neck squamous cell carcinoma cell line SCC-6
10.3760/cma.j.issn.1002-0098.2013.02.004
- VernacularTitle:超音刺猬信号促进头颈部鳞状细胞癌组蛋白去甲基化酶相关基因表达的研究
- Author:
Xiao-Nan YIN
1
;
Yu-Shi MA
;
Juan DU
;
Zhi-Peng FAN
Author Information
1. 青岛市市立医院口腔科
- Keywords:
Carcinoma,squamous cell;
Histone demethylases;
Sonic hedgehog signaling
- From:
Chinese Journal of Stomatology
2013;48(2):77-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether the sonic hedgehog (Shh) signaling could regulate the expression of histone demethylases in the head and neck squamous cell carcinoma(SCC).Methods Human recombinant SHH-N protein or over-expression of the mutant 2 smoothened (M2-SMO) was applied to activate the Shh signaling in tongue squamous cell carcinoma cell line-SCC-6 in this study.Cyclopamine was used to block the Shh signaling in SCC-6.The real-time reverse transcription (RT)-PCR was used to detect the expression of histone demethylases at the mRNA level.Results The data showed that activation of the Shh signaling up-regulated the expression of histone demethylase,lysine-specific demethylase 8 (KDM-8) at the mRNA level by human recombinant SHH-N protein (1.841 ～ 3.591 fold compare with untreated group;P ＜0.01),over-expression of the M2-SMO also increased the expression of KDM-8 (1.358 ～ 3.013 fold compared with empty vector group ;P ＜ 0.05),and after the Shh signaling was blocked by Cyclopamine,the expression of KDM-8 was down regulated (decreased 25.6％ ～ 66.6％ compared with control cells,P ＜0.05).Conclusions Histone demethylase KDM-8 was downstream target gene of Shh signaling in head and neck squamous cell carcinoma cell line SCC-6,and its expression was positively regulated by the Shh signaling.
