High Glucose Promotes the CTGF Expression in Human Mesangial Cells via Serum and Glucocorticoid-induced Kinase 1 Pathway
10.1007/s11596-008-0504-z
- Author:
WANG QUANSHENG
1
;
ZHANG ALI
;
LI RENKANG
;
LIU JIANGUO
;
XIE JIWEN
;
DENG ANGUO
;
FENG YUXI
;
ZHU ZHONGHUA
Author Information
1. Department of Integrated Chinese Medicine and Western Medicine,Wuhan 430022, China
- Keywords:
high glucose;
serum and glucocorticoid-induced protein kinase 1;
human mesahgial cells;
connective tissue growth factor;
diabetic nephropathy
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(5):508-512
- CountryChina
- Language:Chinese
-
Abstract:
Summary: The role of serum and glucocorticoid-induced kinase 1 (SGK1) pathway in the connective tissue growth factor (CTGF) expression was investigated in cultured human mesangial cells (HMCs) under high glucose. By using RT-PCR and Western blot, the effect of SGK1 on the CTGF expression in HMCs under high glucose was examined. Overexpression of active SGK1 in HMCs transfected with pIRES2-EGFP-S422D hSGK1 (SD) could increase the expression of phosphorylated SGK1 and CTGF as compared with HMCs groups transfected with pIRES2-EGFP (FP) under high glucose or normal glucose. Overexpression of inactive SGK1 in HMCs transfected with pIRES2-EGFP-K127N hSGK1 (KN) could decrease phosphorylated SGK1 and CTGF expression as compared with HMCs groups transfected with FP under high glucose. In conclusion, these results suggest that high glucose-induced CTGF expression is mediated through the active SGK1 in HMCs.
