In Vitro Cytotoxicity of Polyphosphoester as a Novel Injectable Alveolar Replacement Material
10.1007/s11596-008-0526-6
- Author:
ZHANG ZHIXING
1
;
MAO JING
;
FENG XIANGLI
;
XIAO JIANZHONG
;
QIU JINJUN
Author Information
1. Department of Orthodontics, Tongji Hospital, Wuhan 430022, China
- Keywords:
polyphosphoester;
cytotoxicity;
MTT assay;
Alamar Blue test
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(5):604-607
- CountryChina
- Language:Chinese
-
Abstract:
Summary: The aim of this study was to investigate the in vitro cytotoxieity of polyphosphoester polymer used as a novel injectable alveolar bone substitutes for controlled delivery of tetracycline. Cell culture medium was exposed to the polymer (0.01-10 mg/mL) for 24h. The L-929 mouse fibroblasts were then exposed to the treated cell culture medium for 24h. Finally, cell viability and growth were assessed by using MTT assay and Alamar Blue assay. No significant cytotoxicity of the polyphosphoester against L-929 mouse fibroblasts was observed at a concentration up to 10 mg/mL (P0.05). The two evaluation methods showed no significant differences (P0.05). This study suggests that polyphosphoester does not demonstrate any significant toxic effects to cells in vitro and has the potential to be used both as a medical device and as scaffolds in tissue engineering applications.
