Inhibitory Effect of Matrine on the Expression of PSA and AR in Prostate Cancer Cell line LNCaP
- Author:
CHEN KE
1
;
HU ZHIQUAN
;
WANG TAO
;
GUO HUI
;
YE ZHANGQUN
Author Information
1. Department of Urology, Tongfi Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China
- Keywords:
prostate cancer;
matrine;
LNCaP cells;
androgen receptor;
prostate specific antigen
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(6):697-699
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the inhibitory effect of matrine on the expression of prostate specific antigen (PSA) and. Androgen receptor (AR) in prostate cancer cell line LNCaP in vitro, LNCaP cells were treated with matrine at different concentrations (0.5, 1.0, 1.5, 2.0 g/L) for 12-36 h. The growth activities of cancer cells were determined by MTI" colorimetric assay, The AR level was measured by Western blotting. The expression of PSA was detected by using AXSYM system-chemical luciferase methods. The results showed that matrine could effectively inhibit the growth of androgen-dependent prostate cancer cell line LNCaP in vitro in a time- and dose-dependent manner (P<0.05). It could obviously decrease the level of AR (P<0.01) and inhibit the expression of PSA in a dose-dependent manner (P<0.05) in LNCaP cells. It was concluded that matrine could significantly suppress the growth of LNCaP cells and inhibit the expression of PSA and AR of prostate cancer cells.
