Effects of Propofol on The mRNA Expression of Toll-like Receptor 4 in BV-2 Cells During Mimic Ischemia-Reperfusion Injury In Vitro
- Author:
ZHANG QING
1
;
SUN PENG
;
ZHANG SHIHAI
;
TIAN YUAN
;
ZHANG JINGHUI
Author Information
1. Department of Anesthesia
- Keywords:
propofol;
Toll-like receptor-4;
ischemia/reperfusion;
microglia
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(6):711-713
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the effects of propofol on the mRNA expression of Toll-like receptor-4 (TLR4) in BV-2 cells during mimic ischemia-reperfusion (I/R) injury in vitro. BV-2 cells, a mouse microglia line, were cultured and divided into 4 groups at random: control group (group C),ischemia/reperfusion group (group I/R), low-dose propofol (25 μmol/L) intervention group (group PF25) and high-dose propofol (100 μmoi/L) intervention group (group PF100). The mRNA expression of TLR4 and NF-kB was measured by means of RT-PCR. TNF-а levels in the supernatants of BV-2 cells were detected by ELISA. The results showed that the mRNA expression of TLR4 and NF-kB was significantly higher in groups I/R, PF25 and PF100 than in group C (P<0.01). And the TNF-а level in the supernatants was elevated in groups I/R, PF25 and PF100 as compared with that in group C (P<0.01). After pre-treatment with propofol, the mRNA expressions of TLR4 and NF-kB and the TNF-ct level were significantly decreased in groups PF25 and PF100 in comparison to those in group I/R (P<0.01). And the decrease in those indicators was more significant in group PF100 than in group PF25 (P<0.01). It was concluded that propofol exerted brain-protecting effects during I/R injury by suppressing the mRNA expressions of TLR4 and NF-kB and deceasing the TNF-а level.
