Up-regulation of VLDL Receptor Expression and Its Signaling Pathway Induced by VLDL and β-VLDL
- Author:
LIU ZHIGUO
1
;
LI HE
;
LI YINGHONG
;
WANG YAN
;
ZONG YIQIANG
;
FENG YOUMEI
;
FENG ZONGCHEN
;
DENG YAOZU
;
QU SHEN
Author Information
1. 华中科技大学同济医学院
- Keywords:
VLDLR;
signal transduction;
transcriptional regulation
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(1):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Very low density lipoprotein receptor (VLDLR) is thought to participate in the patho-genesis of atherosclerosis induced by VLDL and β-VLDL. The present study was undertaken to elu-cidate the effects of VLDL and β-VLDL on VLDLR expression and its signaling pathway. RAW264.7 cells were incubated with VLDL and β-VLDL. The expression of VLDLR mRNA was detected by RT-PCR. The transcriptional activity of VLDLR gene was detected in recombinant plasmid pGL4.2VR-luciferase transfected RAW264.7. Western blot assay was used to detect the changes of phosphorylated ERK1/2 protein. Inhibitors or activators were used to observe the signal pathway in-volving VLDLR expression regulation. The results showed that VLDL and β-VLDL stimulated ERKI/2 activity in a PKC-dependent manner. VLDL or β-VLDL-induced VLDLR expression on macrophages was extremely abolished by inhibitors ERKI/2 or PKC. Our findings revealed that VLDL or β-VLDL-induced VLDLR expression via PKC/ERK cascades and the effect was linked to the transcriptional activation of VLDLR gene promoter.
