Induction of Apoptosis of Human Colon Cancer Cells by siRNA Recombinant Expression Vector Targeting Survivin Gene
- Author:
CAI MING
1
;
WANG GUOBIN
;
TAO KAIXIONG
;
CAI CHANGXUE
Author Information
1. 华中科技大学同济医学院附属协和医院
- Keywords:
.Survivin;
RNA interference;
gene therapy
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(1):45-49
- CountryChina
- Language:Chinese
-
Abstract:
This study constructed siRNA recombinant expression vector targeting survivin gene and observe the apoptosis induction effect of it in human colon cancer cells,siRNA recombinant expres-sion vector targeting survivin gene was constructed and transfected into human colon cancer cells.The effect of siRNA recombinant expression vector was detected by RT-PCR,Western blot,MTT re-duction assay and flow cytometry.It was confirmed by restriction endonuclease and sequence analy-sis that siRNA recombinant expression vector targeting survivin gene was constructed successfully.Inhibition rate of survivin siRNA at mRNA and protein levels was 36.33% and 44.65% respectively.Growth of cancer cells was inhibited and the apoptosis rate was (17.24±2.13)%.The siRNA recom-binant expression vector targeting survivin gene has been constructed successfully.It not only can in-hibit the expression of survivin gene,but also can induce apoptosis in human colon cancer cells re-markably.
