The role of peroxisome proliferators-activated receptor gamma (PPARgamma) during activation of hepatic stellate cells.
- Author:
Wen-zhuo YANG
1
;
Rui-lin LIU
;
Min-de ZENG
;
Lun-gen LU
;
Xi-mei CHEN
;
Shu-chang XU
;
Yi-min MAO
;
Zhu-ping FAN
;
Zhi-rong WANG
;
Ai-ping CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Liver; cytology; Liver Cirrhosis; etiology; pathology; Male; RNA, Messenger; analysis; Rats; Rats, Wistar; Receptors, Cytoplasmic and Nuclear; physiology; Transcription Factors; physiology
- From: Chinese Journal of Hepatology 2004;12(4):216-218
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo observe the role of PPARgamma during the activation process of hepatic stellate cells (HSC).
METHODSBy morphology and RT-PCR, we study the changes of expression of PPARgamma in culture-activated HSC or in vivo activated HSC induced by dimethylnitrosamine (DMN).
RESULTSIn vitro, the expression level of PPARgamma in freshly isolated HSC (0.72+/-0.01) significantly reduced to 0.48+/-0.03 on the third day of culture (t = 19.8372, P<0.01), and reduced 70% on the seventh culture-day and could not be detected after the second passage. In vivo, HSC freshly isolated from normal control rats expressed PPARgamma (0.76+/-0.01). During the development of rat liver fibrosis induced by DMN, the expression level significantly reduced to 0.46+/-0.02 after the third injection of DMN (t = 29.5318, P<0.01), and reduced 66% on the end of first week and could not be detected on the end of second and third week.
CONCLUSIONThe expression of PPARgamma might play an important role on the maintenance of resting-form of HSC, and the reduction of expression of PPARgamma might be an early event during the activation process of HSC.
