Preliminary study on proteomic patterns in hepatic tissue to identify HBV related hepatocellular carcinoma.
- Author:
Yun-zhu LONG
1
;
Xue-gong FAN
;
Ning LI
;
Yu-kun HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; diagnosis; etiology; Hepatitis B; complications; Humans; Liver; chemistry; Liver Neoplasms; diagnosis; etiology; Neoplasm Proteins; analysis; Proteomics
- From: Chinese Journal of Hepatology 2004;12(4):231-233
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify proteomic patterns in hepatic tissues for diagnosing early HBV related HCC.
METHODSProteomic spectra were generated by two-dimensional gel electrophoresis (2-DE), A preliminary "raining" set of spectra derived from analysis of 14 cancer tissues and 14 non-cancer tissues, a proteomic patterns that completely discriminated cancer from non-cancer was identified. The discovered pattern was then used to classify an independent set of 48 masked samples: 24 from cancer tissues, and 24 from non-cancer tissues.
RESULTSThe discriminatory pattern correctly identified all cancer tissues and non-cancer tissues in the masked set. This result yielded a sensitivity of 100%, specificity of 100%.
CONCLUSIONFurther analysis on these proteins in the proteomic pattern will be helpful to screen tumor markers for HBV related HCC. These findings justify a prospective assessment of proteomic pattern technology as a screening tool for cancer in high-risk and general populations.
