Cytokine gene polymorphisms in Chinese patients with type I autoimmune hepatitis.
- Author:
Xiong MA
1
;
De-kai QIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Hepatitis, Autoimmune; immunology; Humans; Interleukin-10; genetics; Middle Aged; Polymorphism, Genetic; Promoter Regions, Genetic; Tumor Necrosis Factor-alpha; genetics
- From: Chinese Journal of Hepatology 2004;12(5):296-298
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the role of Tumor necrosis factor alpha (TNF-alpha) and interleukin 10 (IL-10) gene polymorphisms in susceptibility to type I autoimmune hepatitis (AIH).
METHODSWe detected 2 polymorphisms in TNF-alpha promoter gene at positions -238 and -308 and 3 polymorphic sites in IL-10 gene promoter at positions -1082, -819, -592 by polymerase chain reaction and dot blot with probes in the patients with 32 type I AIH and 48 health controls.
RESULTSGenotypes associated with guanine to adenine substitution at position -308 of TNF-alpha promoter gene occurred more commonly in the patients than in health controls (53.1% vs. 27.1%, RR = 3.05, P < 0.01). There is no significant difference in polymorphisms of TNF-alpha gene at position -238 and 3 polymorphic sites in IL-10 promoter gene between patients and health controls.
CONCLUSIONGenotypes associated with guanine to adenine substitution at position -308 of TNF-alpha promoter gene (TNF-308A) may involve in the pathogenesis of type I AIH
