Rapid detection of the genotyping of hepatitis C virus using DNA chip with coloration methods.
- Author:
Yi-guan ZHANG
1
;
Hong-ju MAO
;
Shi-min GU
;
Bao-jian XU
;
Jian-long ZHAO
;
Zi-ming DONG
Author Information
- Publication Type:Journal Article
- MeSH: 5' Untranslated Regions; genetics; Base Sequence; Genotype; Hepacivirus; classification; genetics; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Sequence Analysis, DNA
- From: Chinese Journal of Hepatology 2004;12(5):301-303
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a new DNA chip with coloration, which can be used for rapid and economical detection of the genotyping of hepatitis C virus (HCV).
METHODSProbes and primers were designed according to the sequence of HCV 5' non-coding region (5' NCR) to fabricate DNA chip. Experimental group consisted of 60 positive serum samples and control group consisted of 20 negative serum samples. To obtain the aimed gene, then they were hybridized with DNA chip. Finally, the results showed in a nylon film. The results of DNA sequencing of samples were used as the control in double blind experimental.
RESULTSUsing DNA chip, HCV was detected in positive of all serum specimens of experimental group and negative in control group. The determination of HCV genotype by DNA chip showed corresponding rate of 96.7% with those by sequence assay.
CONCLUSIONIt showed higher specialty and sensitivity using DNA chip to detect the genotype of HCV. It would be valuable for the clinical genotyping of HCV
