Perforin and granzyme B expression in liver allograft rejection.
- Author:
Jun JI
1
;
Ying-yan YU
;
Hao CHEN
;
Xia-xing DENG
;
Bo-yong SHEN
;
Guang-wen ZHOU
;
Cheng-wen PENG
;
Hong-wei LI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Biomarkers; Female; Graft Rejection; diagnosis; metabolism; Granzymes; Humans; Liver Cirrhosis; surgery; Liver Neoplasms; surgery; Liver Transplantation; immunology; Male; Membrane Glycoproteins; biosynthesis; genetics; Middle Aged; Perforin; Pore Forming Cytotoxic Proteins; Serine Endopeptidases; biosynthesis; genetics; T-Lymphocytes, Cytotoxic; enzymology
- From: Chinese Journal of Hepatology 2004;12(6):325-327
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of perforin and granzyme B in rejection response following liver transplantation, and evaluate their roles to be used as predictive markers of rejection.
METHODSThe expression of perforin and granzyme B in liver biopsies obtained from liver allograft recipients was determined by immunohistochemistry. Biopsies were classified into two groups-no evidence of rejection and rejection-according to Histopathologic criteria. The relationship between the perforin/granzume B expression and acute rejection was analyzed.
RESULTSFrom 19 patients, thirty-five liver biopsies were obtained after liver transplantation. Among them, nineteen samples were diagnosed as rejection response. The frequencies of perforin and granzyme B expression in rejection group were 100% (19/19) and 94.7% (18/19), respectively. While those in no rejection group were 25.0% (4/16) and 12.5 (2/16), respectively. In most rejected samples, perforin and granzyme B were expressed simultaneously. Only three samples showed perforin expression alone, while no samples demonstrated granzyme B expression alone. There was a close relationship between perforin/granzyme B expression and liver allograft rejection.
CONCLUSIONPerforin and granzyme B expression seemed to be related to the development of acute rejection following liver transplantation, and might be served as sensitive and reliable markers in diagnosing acute rejection in early stage.