Immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen.
- Author:
Yi-ping XING
1
;
Zu-hu HUANG
;
Shi-xia WANG
;
Jie CAI
;
Jun LI
;
Shan LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Hepatitis B Antibodies; blood; Hepatitis B Core Antigens; genetics; immunology; Hepatitis B Vaccines; immunology; Immunity, Cellular; Mice; Mice, Inbred BALB C; T-Lymphocytes, Cytotoxic; immunology; Vaccines, DNA; immunology
- From: Chinese Journal of Hepatology 2004;12(6):338-340
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo observe immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen (HBcAg).
METHODSA new DNA vaccine (pSW3891/HBc) encoding for hepatitis B virus core antigen was constructed using plasmid pSW3891 which can be used in human. Control and experiment groups of Balb/c mice were immunized with pSW3891 or pSW3891/HBc by gene gun. Anti-HBc in sera of mice was tested by ELISA (enzyme linked immune sorbent assay). Specific cytotoxicity of cytotoxic T lymphocyte (CTL) of mice was detected by LDH release assay.
RESULTSpSW3891/HBc can express in 293T cell line effectively. Mice immunized with pSW3891/HBc showed strong anti-HBc response and specific high cytotoxicity of CTL.
CONCLUSIONpSW3891/HBc induced significantly humoral and cellular immune responses in Balb/c mice.
