Hypoxia inducible factor 1-alpha mRNA expression in alcoholic liver disease.
- Author:
Shao-Hua CHEN
1
;
Chao-Hui YU
;
You-Ming LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; DNA-Binding Proteins; biosynthesis; genetics; Hypoxia; complications; metabolism; Hypoxia-Inducible Factor 1; Hypoxia-Inducible Factor 1, alpha Subunit; Liver Diseases, Alcoholic; complications; metabolism; pathology; Male; Nuclear Proteins; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Transcription Factors; biosynthesis; genetics
- From: Chinese Journal of Hepatology 2004;12(7):417-419
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hypoxia on chronic alcoholic liver disease.
METHODSTwenty four male Sprague-Dawley rats were randomly into two groups. The alcohol group (n=12) was fed 56% (v/v) of ethanol once per day by gastric infusion at 8 g/kg body weight for 24 weeks. The control group (n=12) was gastrically infused with normal saline with the same dose. At the end of 24 weeks, a blood sample was collected for determination of hepatic enzymes and then the rat was killed. Liver specimens were obtained for immunohistochemical staining and frozen at -80 degrees C used for RT-PCR.
RESULTSSerum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity increased significantly compared to the control group. A significant elevation in the expression of HIF1-alpha in liver of alcohol group was found compared to the control group.
CONCLUSIONHypoxia inducible factor 1-alpha expression was activated by ethanol-induced injury. This information suggested that hypoxia was involved in mechanism of alcoholic liver disease.
