Comparison of human cord blood mesenchymal stem cell culture between using human umbilical cord plasma and using fetal bovine serum.
- Author:
Yan DING
;
Zhiyong LU
;
Yahong YUAN
;
Xiaoli WANG
;
Dongsheng LI
;
Yi ZENG
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Cell Culture Techniques;
Cells, Cultured;
Culture Media;
chemistry;
Fetal Blood;
chemistry;
Humans;
Mesenchymal Stromal Cells;
cytology;
Plasma;
chemistry;
Serum;
chemistry
- From:
Journal of Biomedical Engineering
2013;30(6):1279-1282
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether human umbilical cord plasma (HUP) can be used to culture human cord blood mesenchymal stem cells (HUCMSCs), we collected 20 surplus HUP. After being treated with salting out and diasysis, the HUP were used to culture HUCMSCs as 10% volume, and compared with fetal bovine serum (FBS). Morphological characteristics, growth curve and reproductive activity of HUCMSCs cells were observed. The concentration of bFGF and noggin secreted by HUCMSCs cultured with HUP and FBS medium were detected by ELISA. It was found that compared to FBS, the morphology, reproductive activity and characteristic of HUCMSCs cell cultured with HUP were not distinctively different from FBS. The concentration of bFGF in HUP group was significantly higher than that of FBS group, and the concentration of noggin was also different in the two groups. So we concluded that HUP could be used to culture HUCMSCs for a long-time, and the HUP mediumcoild could be more suitable for the culture of human embryonic stem cell (hESC).
