A comparative study on inducing non-homologous mesenchymal stem cells to differentiate into neural stem cells using non-homologous cerebrospinal fluid.
- Author:
Chao REN
;
Xiaoyun LIU
;
Meirong WAN
;
Deqin GENG
;
Wei GE
;
Jinmei LI
;
Weiwei ZHANG
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cerebrospinal Fluid;
chemistry;
Culture Media;
chemistry;
Flow Cytometry;
Humans;
Immunohistochemistry;
Mesenchymal Stromal Cells;
cytology;
Neural Stem Cells;
cytology
- From:
Journal of Biomedical Engineering
2013;30(6):1290-1297
- CountryChina
- Language:Chinese
-
Abstract:
In order to set up a base for stem cells to be widely used in clinical medicine, we tried to optimize, in this study, the technique that induces human mesenchymal stem cells (hMSCs) to differentiate into neural stem cells by using cerebrospinal fluid (CSF) from the different groups. After the induction, presence of neural stem cells was confirmed with microscope observation, flow cytometry analysis, immunohistochemistry and fluorescent immunohistochemistry. At the same time, we also compared and analysed the data of the number of stem cells when it totally met the requirements for clinical treatment and the days required. At last, we confirmed that hMSCs could be induced to differentiate into neural stem cells, and that the number of cells totally met the requirements for clinical treatment. But there were some differences both in the number of cells and the days required. Among the groups, the group that marrow mesenchymal stem cells from patients own induced by CSF from healthy volunteers used the shortest time and the quantity of the cells was significantly higher than those of the others.
