Mechanisms of sorafenib induced NB4 cell apoptosis.

Yun-jie Zhang; Xin Liu; Yan-ping Song; Gang-can LI; Nai-cen Zhou; Hao Wang; Qi-xia Wang; Jia Xie; Guang LI; Jing-jing Ren; Fei Gao; Xiao-bo Zhang; Jin-qian Dai; Lu Wang; Jiao MU

Return

Mechanisms of sorafenib induced NB4 cell apoptosis.

Author: Yun-Jie ZHANG ^{1
,

2} ; Xin LIU ³ ; Yan-Ping SONG ⁴ ; Gang-Can LI ⁴ ; Nai-Cen ZHOU ⁵ ; Hao WANG ⁴ ; Qi-Xia WANG ⁴ ; Jia XIE ⁴ ; Guang LI ⁴ ; Jing-Jing REN ⁴ ; Fei GAO ⁴ ; Xiao-Bo ZHANG ⁴ ; Jin-Qian DAI ⁴ ; Lu WANG ⁴ ; Jiao MU ⁴
Author Information

1. Department of Hematology, The First Affiliated Hospital, Xi'an Jiaotong University Medicial College, Xi'an 710061, Shaanxi Province, China
2. Xi'an Institute of Hematology, The Central Hospital of Xi'an, Xi'an 710003, Shaanxi Province, China.
3. Department of Hematology, The First Affiliated Hospital, Xi'an Jiaotong University Medicial College, Xi'an 710061, Shaanxi Province, China. E-mail 1iuxin59221@medmail.com.cn.
4. Xi'an Institute of Hematology, The Central Hospital of Xi'an, Xi'an 710003, Shaanxi Province, China.
5. Department of Hematology, The First Affiliated Hospital, Xi'an Jiaotong University Medicial College, Xi'an 710061, Shaanxi Province, China.
Publication Type:Journal Article
MeSH: Antineoplastic Agents; Apoptosis; Caspase 3; Caspase 8; Cell Line, Tumor; Down-Regulation; Humans; Leukemia, Promyelocytic, Acute; Niacinamide; analogs & derivatives; Phenylurea Compounds; T-Lymphocytes, Helper-Inducer
From: Journal of Experimental Hematology 2015;23(1):77-82
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effects of sorafenib on human acute promyelocytic leukemia cell NB4 and its mechanism.
METHODSThe human acute promyelocytic leukemia cell NB4 was treated with different concentrations (0, 1.5, 3, 6 and 12 µmol/L) of sorafenib, the proliferation inhibitory rate of NB4 cells was assayed by MTT, the apoptosis of NB4 was determined with flow-cytomatry after treatment; after extraction of total protein, the Western blot was performed to determine the expressions of apoptosis-relatived molecules Caspase-3, Caspase-8 and MCL-1. The mRNA expressions of Caspase-3, Caspase-8 and MCL-1 were determined by RT-PCR.
RESULTSAs compared with the control group, the proliferation of NB4 significantly decreased after treatment with different concentrations of sorafenib. The sorafenib significantly induced the apopotosis of NB4 cells in time- and dose-dependent manners. Furthermore, sorafenib treatment resulted in the obvious increase of the Caspase-3 and Caspase-8 protein and mRNA expressions, and down-regulated the MCL-1 protein and mRNA expressions in NB4 cells.
CONCLUSIONSorafenib can inhibit proliferation and induce apopotosis of human acute promyelocytic leukemia cell NB4 through the expression of Caspase-3 and Caspase-8, and down-regulation of the expression of MCL-1.