- Author:
Ke-Hui SHI
1
;
Hong-Li JIANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; DNA; Disease Models, Animal; Genetic Therapy; Interleukin-6; Liposomes; Mice; Multiple Myeloma; Oligodeoxyribonucleotides; Transfection
- From: Journal of Experimental Hematology 2015;23(1):132-136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe present study was to evaluate the effects of nuclear factor of kappa B decoy oligodeoxynucleotides on murine multiple myeloma models.
METHODSThe severe combined immunodeficient mice were injected subcutaneously with RPMI-8226 myeloma cells. When tumors became measurable, the mice were divided into 2 treatment groups who respectively received 5 µg/g or 10 µg/g liposome-NF-κB decoy ODN compounds, and one control group was selected; the control group received 10 µg/g liposome-NF-κB mutant decoy ODN compounds, twice per week for 4 weeks. The mice were killed when they died or the tumor diameter became >2 cm.
RESULTSThe liposome-NF-κB decoy ODN could efficiently suppress NF-κB DNA binding activity and inhibited the expression of IL-6. As compared with the control group, the two liposome-NF-κB decoy ODN-treated groups showed more remarkably survival time and smaller tumor volume.
CONCLUSIONIn vivo transfection of NF-κB decoy ODN may provide a new therapeutic strategy for multiple myeloma.