Change and clinical significance of peripheral blood T-lymphocyte functional subsets in acute graft-versus-host disease.
- Author:
Zhi-Rui YANG
1
;
Hai-Yan ZHU
2
;
Wan-Ming DA
1
;
Chun-Ji GAO
1
;
Li YU
1
;
Li-Ye FU
1
;
Meng LI
1
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Flow Cytometry; Graft vs Host Disease; Humans; T-Lymphocyte Subsets
- From: Journal of Experimental Hematology 2015;23(1):190-194
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study was aimed to detect the change of T-lymphocyte functional subsets marked by CCR7 and CD45RA in the aGVHD within 100 days after allo-HSCT and to explore its clinical significance.
METHODSThe peripheral blood of 42 patients after allo-HSCT was collected every two weeks since hematopoietic reconstitution. The expression of CD3, CD4, CD8, CCR7 and CD45RA-marked T-lymphocytes was detected by flow cytometry, the relationship between their expression and the prognosis of aGVHD was analyzed.
RESULTSThe percentage and the absolute count of CCR7(+) T lymphocyte were significantly reduced in aGVHD. The percentage of T(naïve), T(CM), T(EM) and the absolute count of T(naïve), T(EM), TTD were sharply reduced in aGVHD, moreover has changed correspondingly with outcome of aGVHD. The percentage of CD3, CD4, CD8-marked T-lymphocyte subsets did not significantly changed.
CONCLUSIONT-lymphocyte functional subsets marked by CCR7 and CD45RA are a valuable indicator to monitor early immune reconstruction for patients with the aGVHD after allo-HSCT.
