Effect of umbilical cord blood mesenchymal stem cells on peripheral blood lymphocyte subsets.
- Author:
Zhi-Juan XU
1
;
Li-Xia SHENG
1
;
Gui-Fang OUYANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Coculture Techniques; Fetal Blood; Humans; Lymphocyte Count; Mesenchymal Stromal Cells; Stem Cells; T-Lymphocyte Subsets
- From: Journal of Experimental Hematology 2015;23(1):207-211
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study was aimed to investigate the effect of mesenchymal stem cells (MSCs) on subsets and cytokine secretion of T lymphocytes.
METHODSUmbilical cord blood-derived mesenchymal stem cells (UCBMSCs) were isolated by density gradient and were cultared by amplifying culture. The subsets and cytokine secretion of T lymphocytes were detected by flow cytomety after being co-cultured with UCBMSC.
RESULTSThe proliferation of lymphocytes was inhibited. CD4(+)T cell subsets were increased, CD8(+)T cell subsets decreased when co-cultured with UCBMSC; Th1 and Tc1 level significantly reduced, while Th2 and Tc2 level slightly increased.
CONCLUSIONThe UCBMSC can inhibit the proliferation of lymphocytes, especially CD8(+)T cell subsets. In addition, UCBMSCs can reduce Th1 and Tc1 cells, and increase Th2 and Tc2 cells. UCBMSC may have the clinical application potential for preventing and remedying GVHD.
