Comparison of the Tumor-forming Rate between the SCID and NOD/SCID Mice used to Set up Acute Myeloid Leukemia Model.

Hong Yang; Jian-xin Liu; Ze-ping Zhou; Lin Liu; You Zhang

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Comparison of the Tumor-forming Rate between the SCID and NOD/SCID Mice used to Set up Acute Myeloid Leukemia Model.

Author: Hong YANG ¹ ; Jian-Xin LIU ² ; Ze-Ping ZHOU ¹ ; Lin LIU ¹ ; You ZHANG ³
Author Information

1. Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China.
2. Kunming Medical University, Kunming 650101, Yunnan Province, China.
3. Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China. E-mail: youzhangmd@aliyun.am.
Publication Type:Journal Article
MeSH: Animals; Disease Models, Animal; Flow Cytometry; HL-60 Cells; Humans; Leukemia, Myeloid, Acute; Mice; Mice, Inbred NOD; Mice, SCID
From: Journal of Experimental Hematology 2015;23(2):328-334
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo compare the tumor-forming rate between the SCID and NOD/SCID mice to set up acute myeloid leukemia (AML) mouse model.
METHODSThe SCID and NOD/SCID mice were injected with HL-60 cells in to the abdominal cavity in order to contruct the AML mouse model. The gereral status of mice was observed, the positive rate of CD33 in bone marrow cells was detected by flow cytometry, the mouse model was identified by pathologic examination.
RESULTSThe tumor-forming rate in constructed model using SCID mouse was 30%, while the tumor-forming rate in constructed model using NOD/SCID mouse was 100%.
CONCLUSIONCompared with SCID mice, the tumor-forming rate in NOD/SCID mice injected with HL-60 cells is high, the incidence of AML is stable, suggesting that the NOD/SCID mouse model is more suitable to explore the pathogenesis of leukemia.